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Image Search Results
Journal: Frontiers in Immunology
Article Title: Francisella tularensis -infected human neutrophils are trojan horses for infection of macrophages
doi: 10.3389/fimmu.2025.1632942
Figure Lengend Snippet: Differential effects of aged and infected PMNs on intracellular M1 markers and cytokine secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
Article Snippet: Each supernatant was clarified by centrifugation and then used to probe
Techniques: Infection, Control, Enzyme-linked Immunosorbent Assay