proteome profiler human cytokine array kit a slides Search Results


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R&D Systems proteome profiler human cytokine array kit a slides
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Becton Dickinson glass chamber slides coated with matrigel
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
Glass Chamber Slides Coated With Matrigel, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology polyclonal rabbit anti human il 17
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
Polyclonal Rabbit Anti Human Il 17, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioss cd16 polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss collagen 7 polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss bs-0021m
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss pge2 polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss 8-ohdg polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss cdc2/cdk1 polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Bioss prrsv m protein polyclonal antibody
Differential effects of aged and infected PMNs on intracellular M1 markers and <t>cytokine</t> secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Image Search Results


Differential effects of aged and infected PMNs on intracellular M1 markers and cytokine secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Journal: Frontiers in Immunology

Article Title: Francisella tularensis -infected human neutrophils are trojan horses for infection of macrophages

doi: 10.3389/fimmu.2025.1632942

Figure Lengend Snippet: Differential effects of aged and infected PMNs on intracellular M1 markers and cytokine secretion. (A) Lysates of M0, M1 and M2 macrophages and M1 macrophages that ingested aged or infected PMNs (upper left and lower left blots) or M1 macrophages directly infected with F. tularensis (upper right) were probed to detect TGM2, IDO and SOCS1 with β-tubulin as the loading control. Data from three independent experiments are shown (n1-n3). (B-E) Secretion of TNFα (B) , MIP-1α (C) , IL-18 (D) and IL-1β (E) were measured by ELISA. Data are the mean ± SD from three independent experiments and were analyzed by two-way ANOVA with Tukey’s multiple comparisons post-test. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Each supernatant was clarified by centrifugation and then used to probe Proteome Profiler Human Cytokine Array Kit A slides (R&D Systems) according to the manufacturer’s instructions.

Techniques: Infection, Control, Enzyme-linked Immunosorbent Assay